Cloning strategies using a third irrelevant enzyme site (TIES) to overcome certain cloning problems.
نویسندگان
چکیده
We describe a cloning strategy, third irrelevant enzyme site (TIES), based on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES strategy has made it possible to obtain the desired recombinant where the corresponding approach using conventional recombinant DNA methodologies and two-component ligations failed to give the desired product.
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ورودعنوان ژورنال:
- BioTechniques
دوره 20 1 شماره
صفحات -
تاریخ انتشار 1996